Journal: Scientific Reports

Article Title: Co-cultivation of the marine sponge Halichondria panicea and its associated microorganisms

doi: 10.1038/s41598-019-46904-3

Figure Lengend Snippet: ( a ) Schematically diagram of sponge-bacteria co-cultivation set-up. The bottom chamber of a Bio-Dot SF microfiltration apparatus was filled with marine agar and each of the 48 chambers was inoculated with diluted bacterial suspension retrieved from a wild H. panicea specimen. These bacterial inoculations were separated from the top part of the apparatus through a 0.2 µm membrane and 25 µm filter paper. A sponge explant was placed into each of the 48 wells in the top of the apparatus, the device was clamped together and placed into a seawater aquarium for 10 weeks. ( b ) Sponge explants in co-cultivation device at day 1 and after week 10. Sponge explants collected for 16S rRNA gene amplicon sequencing and deceased explants were not replaced after the first week of the experiment. The co-cultivation experiment was conducted once during this study.

Article Snippet: The bottom chamber of a Bio-Dot SF microfiltration apparatus was filled with marine agar and each of the 48 chambers was inoculated with diluted bacterial suspension retrieved from a wild H. panicea specimen.

Techniques: Bacteria, Suspension, Membrane, Amplification, Sequencing